TGFβ signaling in neurons, oligodendrocytes and endothelial cells is unchanged by stroke. A.) Co-localization of pSmad2 (marker of TGFβ signaling) with Millimark Pan-Neuronal antibody cocktail (marker of neurons) See additional file 1 for a 3D projection. B.) Co-localization of pSmad2 with CAII (oligodendrocyte marker) See additional file 2 for a 3D projection. C.) Co-localization of pSmad2 with β-dystroglycan (endothelial cell marker) See additional file 3 for a 3D projection. Images (40×) are from young females at day 3 post stroke but are representative of all time points for both young and old, male and female mice.