Figure 4
Immunoreactivity (IR) and western blotting for Iba1 in the brainstem and spinal cord. A representative blot of Iba-1 is shown significant reduction of Iba I in the brainstem and spinal cord of BV-treated hSOD1G93A mice (A). This experiment was performed using saline- or BV-treated hSOD1 G93A mice (n = 3). Quantitative analysis of immunoblot. The image is representative of three independent experiments. The optical density was measured for each band, and values for Iba-1 were compared with tubulin after correcting for the total protein content. The Results of the densitometric quantifications are the means ± SEM of triplicate samples. The data were analyzed using a t-test. *p < 0.01 versus the corresponding saline-treated group. Iba1 IR of the lumbar (L4) spinal cord of hSOD1G93A mice treated with saline (B) or BV (C). Boxes indicate high magnification views of the ventral horn region. Iba1 IR of the facial nucleus of the brainstem from saline- (D) or BV-treated transgenic mice (E). Scale bars = 200 μm (B, C). Scale bars = 100 μm (D, E). Cell counts of Iba1-positive cells in the ventral horn region or brainstem of saline- (black columns, n = 3) or BV-treated mice (white columns, n = 4) (F, G). Data are shown as the mean ± SEM. Data were analyzed with a t-test. *p < 0.001. BV: bee venom, BS: brainstem, SP: spinal cord