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Figure 2 | Journal of Neuroinflammation

Figure 2

From: β-Adrenoceptor activation depresses brain inflammation and is neuroprotective in lipopolysaccharide-induced sensitization to oxygen-glucose deprivation in organotypic hippocampal slices

Figure 2

Effect of β1-agonist dobutamine on neuronal cell death following lipopolysaccharide-induced sensitization to OGD in murine hippocampal slice cultures. A. Temporal development of cell damage (mean fluorescence intensity (MFI)) after 15 min oxygen-glucose deprivation (OGD), LPS (1 μg/ml) for 24 h + 15 min oxygen-glucose deprivation (OGD), β1-agonist dobutamine, (5 μM) + 24 h LPS + 15 min OGD and β1-agonist dobutamine (50 μM) + 24 h LPS + OGD. The same culture is shown at repeated time points of recovery. B. Cell death (MFI) after 15 min OGD (hatched bars), 24 h LPS+ 15 min OGD (white bars), dobutamine (5 μM) + 24 h LPS + 15 min OGD (shaded bars) and dobutamine (50 μM) + 24 h LPS + 15 min OGD (dark shaded bars) at repeated points of recovery. Data are shown as means ± SEM with n = 18. LPS+OGD versus OGD, P < 0.001 in the CA1; dobutamine (5 μM) + LPS + OGD versus LPS + OGD, p < 0.01 in the CA1; dobutamine (50 μM) + LPS + OGD versus dobutamine (5 μM) + LPS + OGD, p < 0.001 in the CA1. Repeated-measures ANOVA with post hoc Bonferroni.

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Journal of Neuroinflammation

ISSN: 1742-2094