Figure 6

Effect of α2-agonist clonidine on neuronal cell death following lipopolysaccharide-induced sensitization to OGD in murine hippocampal slice cultures. A. Temporal development of cell damage (mean fluorescence intensity (MFI)) after 24 h LPS + 15 min OGD, clonidine (5 μM) +24 h LPS +15 min OGD and clonidine (50 μM) +24 h LPS + 15 min OGD. The same culture is shown at repeated time points of recovery. B. Cell death (MFI) after 24 h LPS + 15 min OGD (white bars), clonidine (5 μM) +24 h LPS +15 min OGD (shaded bars bars), clonidine (50 μM) +24 h LPS + 15 min OGD (dark shaded bars), at repeated points of recovery. Data are shown as means ± SEM with n = 18. Clonidine(5 and 50 μM) + LPS + OGD versus LPS + OGD, non-significant differences at both concentrations) in the CA1.