Blood vessel counts are increased in both the grey and white matter during EAE relapse. (A) Clinical grade of animals during the different phases of EAE. Animals display neurological impairment during the acute phase and relapse, whereas they show almost complete remission between the two episodes. The acute inflammatory phase ranges from 9 to 17 dpi, the remitting phase from 17 and 21 dpi and relapse from 21 to 26 dpi. (B) Collagen IV immunohistochemistry and subsequent quantification shows a significant increase of blood vessels at day 22 (relapse) in the lumbar spinal cord grey and white matter. There is little change in blood vessel numbers in either the thoracic or cervical spinal cord, with the exception of a small increase in the thoracic white matter at day 22 post immunization. n = 5/rats timepoint mean ± S.D. ° p < 0.05 compared to day 13, * p < 0.05 compared to naive rats.