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Table 1 Antibodies used for immunohistochemistry

From: Angiogenesis is present in experimental autoimmune encephalomyelitis and pro-angiogenic factors are increased in multiple sclerosis lesions

Primary Antibody Source Isotype Species Reactivity* Clone or Reference Dilution Supplier
Angiopoietin-1 Mouse monoclonal IgG1 h, m, r F-1 4 μg/ml Santa Cruz Biotechnology, Santa Cruz, CA
Angiopoietin-1 Rabbit polyclonal IgG h, m, r Ab65835 2 μg/ml Abcam, Oxford, UK
GFAP Rabbit polyclonal IgG h, m, r G560A 1 μg/ml Promega, Madison, WA
Meteorin Rabbit polyclonal IgG h, m Ab56129 2 μg/ml Abcam
Meteorin Goat polyclonal IgG m AF3475 6 μg/ml R&D Systems, Abington, UK
Tie-2 Rabbit polyclonal IgG h, m, r Sc-9026 2 μg/ml Santa Cruz
VEGF Rabbit polyclonal IgG h, m, r sc-152 1 μg/ml Santa Cruz
VEGF Mouse monoclonal IgG1 h, m, r VG-1 3 μg/ml Abcam
VEGFR1 (Flt-1) Rabbit polyclonal IgG h Sc-74007 2 μg/ml Santa Cruz
VEGFR1 Rabbit polyclonal IgG h, m, r Ab2350 1 μg/ml Abcam
VEGFR2 (Flk-1) Rabbit polyclonal IgG h, m, r Ab2349 3 μg/ml Abcam
VEGFR2 Rabbit polyclonal IgG h, m, r Sc-504 2 μg/ml Santa Cruz
Collagen IV Rabbit polyclonal IgG r, m Ab19808 2 μg/ml Abcam
CD31 Rabbit polyclonal IgG h, m Ab78364 2 μg/ml Abcam
  1. * h = human, m = mouse, r = rat