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Table 1 Antibodies used for immunohistochemistry

From: Angiogenesis is present in experimental autoimmune encephalomyelitis and pro-angiogenic factors are increased in multiple sclerosis lesions

Primary Antibody

Source

Isotype

Species Reactivity*

Clone or Reference

Dilution

Supplier

Angiopoietin-1

Mouse monoclonal

IgG1

h, m, r

F-1

4 ÎĽg/ml

Santa Cruz Biotechnology, Santa Cruz, CA

Angiopoietin-1

Rabbit polyclonal

IgG

h, m, r

Ab65835

2 ÎĽg/ml

Abcam, Oxford, UK

GFAP

Rabbit polyclonal

IgG

h, m, r

G560A

1 ÎĽg/ml

Promega, Madison, WA

Meteorin

Rabbit polyclonal

IgG

h, m

Ab56129

2 ÎĽg/ml

Abcam

Meteorin

Goat polyclonal

IgG

m

AF3475

6 ÎĽg/ml

R&D Systems, Abington, UK

Tie-2

Rabbit polyclonal

IgG

h, m, r

Sc-9026

2 ÎĽg/ml

Santa Cruz

VEGF

Rabbit polyclonal

IgG

h, m, r

sc-152

1 ÎĽg/ml

Santa Cruz

VEGF

Mouse monoclonal

IgG1

h, m, r

VG-1

3 ÎĽg/ml

Abcam

VEGFR1 (Flt-1)

Rabbit polyclonal

IgG

h

Sc-74007

2 ÎĽg/ml

Santa Cruz

VEGFR1

Rabbit polyclonal

IgG

h, m, r

Ab2350

1 ÎĽg/ml

Abcam

VEGFR2 (Flk-1)

Rabbit polyclonal

IgG

h, m, r

Ab2349

3 ÎĽg/ml

Abcam

VEGFR2

Rabbit polyclonal

IgG

h, m, r

Sc-504

2 ÎĽg/ml

Santa Cruz

Collagen IV

Rabbit polyclonal

IgG

r, m

Ab19808

2 ÎĽg/ml

Abcam

CD31

Rabbit polyclonal

IgG

h, m

Ab78364

2 ÎĽg/ml

Abcam

  1. * h = human, m = mouse, r = rat