Figure 1
Gp120-mediated increased expression of IL-8 in SVGA astrocyte cells. 1 × 106 SVGA astrocytes were transfected with 1 μg gp120 DNA for 5 hours, using a lipofection method. Total RNA was extracted from cells at 6, 12, 24, 48 and 72 hours after transfection, and culture supernatant was collected at the same times. IL-8 mRNA was determined using real time PCR whereas protein was measured using a Bio-plex method. Expressions of IL-8 at the mRNA (A) level were compared between gp120-transfected cells and those with empty plasmids, and are presented as fold change. (B) IL-8 protein levels are shown as pg/ml culture supernatant and were compared between gp120-transfected and control cells transfected with empty plasmid. Each bar represents mean ± SE for 3 independent experiments, with each experiment done in triplicate. Student's t test was used for statistical analysis and statistical significance is denoted as * (p value ≤ 0.05).