TUNEL staining representing apoptotic cells in and around the penumbra of ipsilateral brain after 24 hrs of reperfusion in all three groups (Sham, Vehicle and H-100). The green staining represents TUNEL-positive cells. Images were taken at 100 × magnification. Scale bar = 80 μm. A graphical representation of TUNEL-positive cell counts, indicated as sham-normalized fold change is also shown; "a" for p ≤ 0.05 when compared to sham group; "b" for p ≤ 0.05 when compared to vehicle group.