Figure 5
TNFα levels in serum and brain of mice following acute inflammation; relevance of TNFα p55 receptor for B 1 receptor mRNA expression. Time-related effects of treatment with LPS (450 μg/kg, i.p.) on TNFα levels in serum (a), whole brain (b) or CSF (c) of CF1 mice, submitted to a previous 5-min forced swimming session, according to assessment by ELISA sandwich (a, b) or CBA (c) assays. (d) Effect of systemic administration of E. coli LPS (1000 μg/kg i.p., 24 h) on immobility time, in C57/BL6 or TNFα p55 receptor knockout mice, as assessed in the tail suspension test. (e) Evaluation of B1 receptor mRNA expression in C57/BL6 or TNFα p55 receptor knockout mice that had been previously submitted to a 5-min swimming section, and were pre-treated with E. coli LPS (1000 μg/kg i.p., 1 h). Each column represents the mean of 6-8 animals and vertical line show the SEM. *P < 0.05, **P < 0.01 compared to naive or vehicle-treated mice and ##P < 0.01 compared to C57BL/6 mice.