Figure 2

Neutrophils in microvascular injury after SAH. A: Representative image showing neutrophils in a brain section from an animal sacrificed 10 min after SAH. Note that some neutrophils (red) are within the collagen IV stained vessels (green; arrow heads) and others are present in the brain parenchyma (arrows). B: Area fractions of collagen IV and RECA-1 immunostaining in SAH and sham animals. Note that the area fraction of collagen IV and RECA-1 staining in SAH animals remained lower than the sham operated animals at all times examined. C: Numbers of neutrophils which are colocalized with collagen IV and RECA-1 after SAH. Filled circles: all neutrophils; filled squares: neutrophils that colocalized with collagen IV only; filled triangles: neutrophils that colocalized RECA-1 only. Open circles show all neutrophils in sham operated animals. Note that a greater number of neutrophils colocalized with collagen IV than with RECA-1 during the first 3 hours after SAH. Data are mean ± sem, N = 5 animals per time point and represent totals per whole coronal brain section * significantly different from sham operated animals (B) or from RECA-1 (C) at p < 0.05.