Figure 6

PPCSE-induced HO-1 expression is mediated via a NADPH oxidase/ROS/PDGFR/PI3K/Akt signaling in bEnd.3 cells. A, B: Cells were pretreated without or with wortmannin (Wort, 1 μM) or AG1296 (3 μM) for 1 h, and then stimulated with 30 μg/ml PPCSE for the indicated time intervals. The cell lysates were analyzed by Western blot using an anti-phospho-PDGFR, anti-phospho-Akt, or anti-GAPDH antibody. C: Cells were pretreated without or with D609 (an inhibitor of PC-PLC), rotterin (an inhibitor of PKCδ), APO (an inhibitor of NADPH oxidase), or NAC (a ROS scavenger) for 1 h, and then stimulated with 30 μg/ml PPCSE for the indicated time intervals. The phosphorylations of Akt and PDGFR were determined by Western blot. Similar results were obtained from three independent experiments.