Figure 6

Immunofluorescence staining for TNF-R2; a) in the fresh, organ culture after 24 h incubation, organ culture after 48 h incubation, 24 h culture + treatment with IMD-0354, 48 h culture + treatment with IMD-0354, 24 h culture + treatment with SB386023-b, 48 h culture + treatment with SB386023-b, 24 h culture+ treatment with U0126 and 48. The expression of TNF-R2 increased in culture with a maximum in 48 h compare to fresh group. UO126 treatment significantly prevented this expression while, both IMD-0354 and SB386023-b slightly reduced the TNF-R2 protein level. b) Bar graph demonstrating the fluorescence intensity for TNF-R2. The results are expressed as mean values ± S.E.M. *P < 0.05 and **P < 0.01, significant difference between fresh and culture after 24 and 48 h. ^§P < 0.05, significant difference between 48 h organ culture and treatment with 48 h culture + U0126. ^#P < 0.05, significant difference between 24 h organ culture and treatment with 24 h culture + U0126. n.s. = not significant.