Mouse WT and Ngr1/2-/- DC characterisation and NgR expression. Expression of cell surface markers on mouse WT (A) and Ngr1/2-/- (KO) (C) bone marrow derived DCs, as quantified with flow cytometry. Bars represent mean of percentage of positive cells for the indicated marker, with SEM of at least 6 experiments (6 of each WT and Ngr1/2-/- mice). *P < 0.05, **P < 0.01. (B) Relative mRNA expression of NgR1, NgR2, LINGO-1, TROY and p75NTR were determined with TaqMan RT qPCR. Mean values relative to immDC with SEM from 9 experiments, representing 9 mice, are shown. Wilcoxon matched-pairs signed rank test was used with delta Ct values to determine significance. *P < 0.05, **P < 0.01. (D) A panel of 32 cytokines were measured in cell culture supernatant from 3 mice of each: WT immDC (WT iDC) and matDC (WT mDC), and Ngr1/2-/- immDC (KO iDC) and matDC (KO mDC). The relative concentrations (as a ratio to the positive control of the assay) of these cytokines are shown as a heatmap. Low concentrations are shown in blue, median concentrations in green and high concentrations in red.