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Table 1 PCR targets

From: Central administration of insulin-like growth factor-I decreases depressive-like behavior and brain cytokine expression in mice

Protein Gene(transcript) Primary Expressing Cells Function Ct Catalog #
TNFα Tnf Microglia Pro-Inflammatory Cytokine 30 Mm00443258_m1
IL-1ß Il1b Microglia Pro-Inflammatory Cytokine 28 Mm00434228_m1
iNOS Nos2 Microglia Nitric Oxide Generation 30 Mm00440485_m1
IL-10 Il10 Microglia, Astrocytes Anti-Inflammatory Cytokine 35 Mm00439616_m1
IL-4 Il4 Microglia Anti-Inflammatory Cytokine 38 Mm00445260_m1
IL-6 Il6 Astrocytes, Microglia Pro-Inflammatory Cytokine 32 Mm00446190_m1
GFAP Gfap Astrocytes Cytoskeletal 18 Mm00546086_m1
BDNF Bdnf (I-IX) Neuronal Soma Growth Factor 26 Mm01334047_m1
BDNF Bdnf (VI-IX) Neuronal Dendrites-Soma > Astrocytes Growth Factor 25 Mm01334042_m1
IGF-I Igf1 (Ea) Neurons, Activated Glia Growth Factor 27 Mm00710307_m1
IGF-I Igf1 (Eb) Neurons, Activated Glia Growth Factor 30 Mm00439561_m1
COX2 Ptgs2 Endothelial cells Prostanoid Synthesis 25 Mm00478372_m1
GAPDH Gapdh All Cells Glycolysis 17 Mm99999915_g1
  1. Gene expression pattern was designed to initiate work to define the mechanism by which IGF-I affects behavior. To do this; steady state expression of a panel of genes were quantified. Although not directly translatable to mRNA levels, average Ct values for control mouse samples are provided for each transcript to give an indication of their relative abundances. Gene expression with Ct values of ≥ 35 were considered as low expression genes (right justified), genes with Ct values between 30 and 34 were of medium expression (center justified, underlined) and those with Ct values of ≤ 29 were highly expressed (left justified, bold-faced).