Central administration of insulin-like growth factor-I decreases depressive-like behavior and brain cytokine expression in mice

Table 1 PCR targets

Protein	Gene_(transcript)	Primary Expressing Cells	Function	Ct	Catalog #
TNFα	Tnf	Microglia	Pro-Inflammatory Cytokine	30	Mm00443258_m1
IL-1ß	Il1b	Microglia	Pro-Inflammatory Cytokine	28	Mm00434228_m1
iNOS	Nos2	Microglia	Nitric Oxide Generation	30	Mm00440485_m1
IL-10	Il10	Microglia, Astrocytes	Anti-Inflammatory Cytokine	35	Mm00439616_m1
IL-4	Il4	Microglia	Anti-Inflammatory Cytokine	38	Mm00445260_m1
IL-6	Il6	Astrocytes, Microglia	Pro-Inflammatory Cytokine	32	Mm00446190_m1
GFAP	Gfap	Astrocytes	Cytoskeletal	18	Mm00546086_m1
BDNF	Bdnf _(I-IX)	Neuronal Soma	Growth Factor	26	Mm01334047_m1
BDNF	Bdnf _(VI-IX)	Neuronal Dendrites-Soma > Astrocytes	Growth Factor	25	Mm01334042_m1
IGF-I	Igf1 _(Ea)	Neurons, Activated Glia	Growth Factor	27	Mm00710307_m1
IGF-I	Igf1 _(Eb)	Neurons, Activated Glia	Growth Factor	30	Mm00439561_m1
COX2	Ptgs2	Endothelial cells	Prostanoid Synthesis	25	Mm00478372_m1
GAPDH	Gapdh	All Cells	Glycolysis	17	Mm99999915_g1

Gene expression pattern was designed to initiate work to define the mechanism by which IGF-I affects behavior. To do this; steady state expression of a panel of genes were quantified. Although not directly translatable to mRNA levels, average Ct values for control mouse samples are provided for each transcript to give an indication of their relative abundances. Gene expression with Ct values of ≥ 35 were considered as low expression genes (right justified), genes with Ct values between 30 and 34 were of medium expression (center justified, underlined) and those with Ct values of ≤ 29 were highly expressed (left justified, bold-faced).

ISSN: 1742-2094