Figure 1

Retinal zones and layers used for quantifying microglia. (A) Retinal flat mount stained with lectin showing the regions used for evaluation. Vascular tufts are located at the border of the central avascular zone and the peripheral zone. (B) Cryosection of the eye showing the superficial layer (s) and the deep layer (d) of microglia (GFP, green). The nuclei (DAPI, blue) of the retinal ganglion cells (RGC), inner nuclear layer (INL) and outer nuclear layer (ONL) are shown for orientation. Macrophages in the choroid (c) and sclera express GFP, too. Vitreous (v).