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Figure 8 | Journal of Neuroinflammation

Figure 8

From: Pro-inflammatory cytokines and lipopolysaccharide induce changes in cell morphology, and upregulation of ERK1/2, iNOS and sPLA2-IIA expression in astrocytes and microglia

Figure 8

sPLA2-IIA immunoreactivity in DITNC and primary astrocytes. (A) DITNC cells were cultured on coverslips and stimulated with cytokines or LPS + IFNγ for 24 h. (B) primary astrocytes were treated with cytokines or LPS + IFNγ for 48 h. After exposure to cytokines and LPS, cells were permeabilized and double-immunostained with GFAP (left, green) and sPLA2-IIA (middle, red) with merged images (right). Scale bar represents 20 μm.

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