BDNF-induced phospho-ERK1/2 in DRG cultures after TNF-α treatment. DRG cultures were treated with 5 nM TNF-α for 48 hours prior to 400 ng/ml BDNF. Protein signals were detected by western immunoblot up to 40 min after BDNF treatment. (A, B) Gel pictures illustrating the phospho-ERK1/2 and β-actin signals in control (no drug treatment) and TNF-α-treated groups, respectively. (C, D) Quantitative measurements of phospho-ERK1 and phospho-ERK2 signals in control and TNF-α-treated groups, respectively. The results are presented as ratios relative to β-actin. The data are expressed as mean ± SEM and were analyzed using one-way ANOVA followed by Tukey test, *p < 0.05, **p < 0.01, ***p < 0.001; compared to time 0. The comparison between basal TNF-α and control groups were analyzed using an un-paired Student's t-test; ##
p < 0.01, ###
p < 0.001, (N = 6 per group).