Figure 7

TNF-α-induced BDNF content and release in DRG cultures. After a 5 nM TNF-α treatment for 24 and 48 hours, medium and lysed cells were collected and analyzed by BDNF EIA. BDNF release represents basal or TNF-α-induced BDNF in the culture medium; BDNF content means basal or TNF-α-induced BDNF remaining in cultured DRG cells. Data are expressed as mean ± SEM and were analyzed using one-way ANOVA followed by Tukey test, *p < 0.05; compared to corresponding basal groups (N = 5 per group).