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Figure 2 | Journal of Neuroinflammation

Figure 2

From: Generation of reactive oxygen species in 1-methyl-4-phenylpyridinium (MPP+) treated dopaminergic neurons occurs as an NADPH oxidase-dependent two-wave cascade

Figure 2

Dopaminergic cells express subunits of the NADPH oxidase complex. (A) mRNA from untreated N27 cells was reverse transcribed and amplified using PCR primers specific to rat NADPH oxidase subunits. Nox1-4 subunits as well as p22phox, p40phox, p47phox, and p67phox were identified by their mRNA expression. Nox2, p22phox, and p47phox were also detected by their protein expression (Western immunoblot, B-D) and cellular localization (immunofluorescence, B-D). Scale bar equals 10 μm; all three micrographs were taken at the same magnification.

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