Figure 17

NFκB expression in primary cultures of AMC labeled by OX-42. NFkB immunoexpression is clearly enhanced in primary culture microglia exposed to LPS (Ba-c) when compared with control AMC (Aa-c). Decreased NFkB immunoexpression in AMC is observed in AMC treated with either DMS alone (Ca-c) or DMS + LPS (Da-c). Co-expression of OX-42 and NFkB is evident in cells as seen in merged images (arrows).Moreover the nuclear translocation of NFκB appears to be more evident in LPS-treated cells compared with control and DMS + LPS treated cells. Nuclei are stained with DAPI. Scale bar = 20 μm.