Figure 2

Surface marker and cytokine expression profile of SB623 cells compared to parental MSCs. A) Plots showing surface marker expression on SB623 cells and MSCs. Both SB623 cells and MSCs have >95% expression of CD44, CD73, and CD105, however, there is an increase in fluorescence intensity (measured by FACS) of these markers in SB623 cells compared to parental MSCs (*p < 0.05, n = 3, left plot); there is consistently higher expression of CD54 in SB623 cells versus matched lots of parental MSCs (right plot); B) Plots showing mean fluorescence intensity of IL-6, GM-CSF, IL-1A, TGFβ1, and VEGF-A (measured by intracellular antibody staining and flow cytometry) for 3 different matched lots of MSC and SB623 cells.