Figure 1
From: Cautionary notes on the use of NF-κB p65 and p50 antibodies for CNS studies
Western blots show p65 antibodies that passed the test of specificity in p65 KO tissues. Amounts of cell extract loaded per lane indicated after each antibody. a, sc-372R (brain 20 μg, spleen 20 μg, liver 20 μg); b, NIH p65 (brain 40 μg, spleen 20 μg, liver 20 μg); c, Epitomics 1546 (brain 20 μg, spleen 20 μg); d, Invitrogen 2A12A7 (neurons 10 μg); e, Cell Signaling 3034 (brain 20 μg, spleen 40 μg); f, Cell Signaling 4764 (brain 40 μg, spleen 40 μg, liver 40 μg); g, Santa Cruz sc-109 (brain 40 μg, spleen 40 μg, liver 40 μg); h, Calbiochem PC137 (brain 20 μg). The pattern of bands seen in h was identical to the patterns produced by the same antibody sold by Chemicon (AB 1604) and Rockland (100-4165) (not shown). Arrows point to locations in blots of the protein identified as p65. WT, wildtype; KO, knockout tissue.