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Table 1 NF-κB Antibodies tested for specificity in p65 KO and p50 KO tissues

From: Cautionary notes on the use of NF-κB p65 and p50 antibodies for CNS studies

Catalog Name

Target

Source

Anti p65 antibodies (C-terminus-directed listed first)

sc-372G/R

Goat or rabbit polyclonal to aa 531-550 (C-20)

Santa Cruz

NIH p65 (7057)

Rabbit polyclonal to aa 538-550

NIH-Siebenlist

PC137

Rabbit polyclonal to C-term (within the last 60 aas) Same as Chemicon (AB 1604), Rockland (100-4165), and Upstate (now Millipore)

Calbiochem

1546

Rabbit monoclonal to C-terminus

Epitomics

2A12A7

Mouse monoclonal to aa 375-550

Invitrogen

3034

Rabbit polyclonal to region around Ser276

Cell Signaling

4764

Rabbit monoclonal to N-terminus

Cell Signaling

sc-109

Rabbit polyclonal to N-terminus

Santa Cruz

sc-8008

Monoclonal to N-terminal aa 1-286

Santa Cruz

"Activated" p65 antibodies

MAB3026

Clone 12H11; monoclonal to region of NLS (formerly Boehringer and Roche #1697838; originally Kaltschmidts' a-p65M)

Chemicon

3037

Rabbit polyclonal to phospho-Ser276-p65

Cell Signaling

2615

Rabbit polyclonal to phospho-Ser276-p65

Abcam

Nancy Rice (NCI, Frederick, MD repository) rabbit polyclonal p65 antibodies

1226-TB3

C terminus

NCI repository

1774 TB11

downstream of NLS

NCI repository

1207-TB4

N terminus

NCI repository

33-TB4

downstream of NLS (mouse sequence)

NCI repository

Anti p50 antibodies

ab7971

Rabbit polyclonal to aa 330-433

Abcam

sc-8414

Monoclonal to aa 120-239

Santa Cruz

06-886

Rabbit polyclonal to aa 1-12

Upstate

3035

Rabbit polyclonal to N-terminal

Cell Signaling

1559

Rabbit monoclonal between aa 340-370

Epitomics

"Activated" p50 antibody

sc-114

Rabbit polyclonal to region of NLS (at 357-365)

Santa Cruz

Nancy Rice (NCI, Frederick, MD repository) rabbit polyclonal p50 antibodies

1263-TB7

N terminus of mouse p50

NCI repository

1157-TB5

upstream of NLS

NCI repository

1613-TB7

NLS

NCI repository

  1. Manufacturers' information about location of amino acid (aa) sequences of the human protein that the antibody was raised against.