Figure 3

Time-dependent PAK1 expression in response to traumatic stress. Rats were killed 1, 3, or 7 days after traumatic stress (n = 5 for each time point). Cross sections of frontal cortex were immunostained for anti-PAK1 antibody (A), and the density of immunopositive cells was semi-quantified in three randomly chosen areas (B). Frontal cortex homogenates were prepared, and IL-1RI expression was determined by ELISA assay (C). Con: control; T1, 3, 7: 1, 3, 7 days after trauma. Data are presented as percentage of control; each value represents mean ± SD for three independent experiments. *P <0.05 vs Con. Scale bars = 50 μm.