Figure 7
c-Src signaling in neuroimmune modulation in traumatized rats. Four groups of rats were prepared as described in Fig. 6: Con, T3, T3+DN c-Src, T3+CA c-Src (n = 5 for each group). Homogenates of spleen were prepared, and lymphocyte proliferation and NK cell activity were assayed by [3H] incorporation (A). Rats were subjected to surgical trauma, and 1 day later some of these rats were treated with PAK-1 Ab or IL-1ra. Thus, 4 groups of rats were created: Controls (Con; no trauma), T1 (rats killed 1 day after surgical trauma), T1+PAK-1 Ab (icv administration of antibody against PAK1, 1 day after trauma and killed 24 hours later), and T1+IL-1ra (icv administration of antibody against IL-1ra 1 day after trauma and killed 24 hours later) (n = 5 for each group). Homogenates of spleen were collected, and lymphocyte proliferation and NK cell activity were assayed by [3H] incorporation (B). Data are presented as percentage of control. Values represent mean ± SD for 3 independent experiments. *p < 0.05 vs Con, #p < 0.05 vs traumatized.