Figure 7

Histological and morphological changes in three regions of brain after PQ (10 mg/kg b.w.) treatment. Morphological alterations in cells were observed by H&E staining in SN, FC and hippocampus of PQ-treated animal brains compared to controls. The detailed procedure for staining is described in Methods. (A): Formation of pyknotic nuclei (black arrows) is found in SN of PQ-treated mouse brain, but not in vehicle-treated controls. (B): Pyknotic nuclei (black arrows) and Lewy body-like structures (yellow arrows) appeared in FC of PQ-treated mouse brain (b, d, f and h) but not in controls (a, c, e and g respectively). Lewy body-like structures of different shapes with acidophilic central cores were found in four regions of frontal cortex. Lewy body-like structures were round-to-oval-shaped (b, h), or irregular-to-pear-shaped (d, f). (C): Pyknotic nuclei were observed in the hippocampal regions dentate gyrus, CA3 and CA1 in PQ-treated animals (b, d, f respectively) but not in control brain (a, c, e respectively). Magnification is 100× as indicated; scale bars = 10µm.