Figure 5

CD4 ⁺ T cell ratios after brain injury were assessed by flow cytometry for CD3 and CD4 labelled cells shown in a representative scatter plot (B). Temporal changes in CD4⁺ T cell ratios after brain injury reveal no significant differences between wild type (solid line) and MT-I/II^-/- mice (dashed line) (A), n = 6-7, error bars = SEM. The CD4⁺ cell gate revealed the ratios of CD25⁺ and FoxP3⁺ naturally occurring regulatory T cells (C). At 3 and 7 DPI no significant differences were observed between wild type mice (grey bars) and MT-I/II^-/- mice (white bars) (D), n = 7, error bars = SEM.