Histopathological findings at different time points from pMCAO. A: representative brain coronal sections obtained 24 h after pMCAO. Pale demarcated areas depict the ischemic lesion. Quantification of ischemic volume (B), neuronal counts (data obtained from the mean of 33 frames/mouse and expressed as % of sham groups, C) and TUNEL-positive cells (D) in the cortex of sham and ischemic mice at different times after pMCAO. Data are reported as mean+sd, n = 8. *p < 0.05, **p < 0.01, ***p < 0.001 versus sham; °p < 0.05, °°p < 0.01, °°°p < 0.001 versus 6 h, Bonferroni's Multiple Comparison Test.