Immunohistochemical analysis and quantification of microglial markers: CD11b, CD45, CD68, Ym1, CD206. A: representative micrographs of CD11b immunostaining in the injured cortex at 24 hours after pMCAo. CD11b-positive cells display different morphology depending on their localization in the ischemic area (C: outside the lesion, D: border zone, E: ischemic core). B: quantification of CD11b immunostaining at different times after pMCAo. Representative micrographs of CD45 (F), CD68 (G), Ym1 (H), CD206 (I) immunoreactivity at 24 hours after ischemia, and related quantifications at different times after pMCAO (Bar 10 μm). Data are expressed as mean+sd of 33 frames/mouse (24 frames/mouse for CD45, TUNEL, CD206), n = 8. One way Anova: p < 0.0001. *p < 0.05, **p < 0.01 vs sham; °°p < 0.01, °°°p < 0.001 vs 6 h (12 h for Ym1). Bonferroni's Multiple Comparison Test.