Figure 7

Coexpression of CD11b (red) and CD206 (green) at 24 h (A-B-E-F) and 7d (C-D-G-H) after pMCAO. At 24 h after ischemia some globular CD11b cells co-label with CD206 marker in the ischemic core (A). Seven days after ischemia CD11b cells are prevalently globular in the ischemic core and are highly positive to CD206 (C). High magnifications (E-G) and 3D rendering (F-H) show colabeling of markers further highlighting the coexpression (blu = nuclei, bar: 5 μm). Consistent with the observation that no CD206 cells are present in the border zone (Figure 4), no immunostaining for this marker at neither time points could be observed in this area (B, D). Data are representative of 3 independent experiments. Bars: 20 μm.