Coexpression of CD68 (red) and Ym1 (green) at 24 h (A-B-E-F) and 7d (C-D-G-H) after pMCAO. At 24 h CD68 positive cells found in the ischemic core do not co-localize with Ym1 positive cells (A). In high magnifications panels (E-F) the two markers appear to belong to different cells although in close contact (blu = nuclei). Seven days after ischemia, when CD68 immunoreactivity is greately increased, Ym1 appears to be expressed also, but not exclusively in CD68 positive cells (C). Note the presence of one Ym1 positive cells (arrow) that does not co-localize with CD68. High magnifications (G) and 3D rendering (H) show colabeling of markers further highlighting the coexpression (blu = nuclei). Consistent with the observation that no Ym1 cells are present in the border zone (Figure 4), no immunoreactivity for this marker could be observed at neither time points in that area (B, D). Data are representative of 3 independent experiments. Bars: 20 μm. High magnifications and 3D rendering bar: 5 μm.