Coexpression of CD68 (red) and CD206 (green) at 24 h (A-B-E-F) and 7d (C-D-G-H) after pMCAO. At 24 h and 7d after ischemia, a minor part of CD68 positive cells found in the ischemic core colocalize with CD206 (A, C). High magnification (E-G) and 3D rendering (F-H) show both single- and double-positive cells in the ischemic core (blu = nuclei, bar: 5 μm). Consistent with the observation that no CD206 cells are present in the border zone (Figure 4), no immunoreactivity for this marker could be observed at neither time points in that area (B, D). Data are representative of 3 independent experiments. Bars: 20 μm.