TUNEL staining after PDT and VEGF inhibition. (A-H) TUNEL (red) and Hoechst (blue) stainings of retina. Eyes were injected with VEGFR1 Fc (2 μg/μl) 7 days before PDT, and retinas were fixed 3 days after PDT (A,B,E,F). Or, VEGFR1 Fc (2 μg/μl) was injected 7 days after PDT, and retinas were fixed 3 days after injection (C,D,G,H). Almost no TUNEL staining is observed in the retina. Scale bar, 100 μm (A-D), 50 μm (E-H).