Figure 5

GPE blocks the expression of inflammatory markers that are induced by LPS. Mice were treated with vehicle or GPE (5 μg i.c.v.) followed 30 min later by vehicle or LPS (330 μg/kg body weight i.p.). Mice were sacrificed, perfused and brains collected 9 h later. Steady-state mRNA expression of pro-inflammatory markers, IL-1β (A), TNFα (B), YM-1 (C) and iNOS (D), was quantified by real-time rtPCR in the prefrontal cortex and expressed relative to GAPDH. GAPDH expression was constant across treatment, p > 0.5, and changes in gene expression shown in Figures 5, 6, and 7 reflect treatment-induced effects on target gene expression. n = 11-12.