Figure 3

Q-PCR validation of the Ad-IRF3 effects in multiple microglial cases. Microglial cultures were transduced with Ad-GFP or Ad-IRF3, treated with IL-1β/IFNγ, and then Q-PCR analysis was performed as described in Figure 2 legend. Data were generated from 5-6 different microglial cases, with each symbol representing a different case. (A) Genes upregulated by IRF3: fold changes in mRNA were calculated as described in the Methods. Fold induction by Ad-IRF3 over Ad-GFP was expressed in a log scale (0.1 = no induction). (B) Genes downregulated by IRF3. Y-axis denotes % inhibition by IRF3 calculated using the formula, 100 × [1 - Ad-IRF3/Ad-GFP]. In both A and B, p-values were generated using single sample t-test (GraphPad Prism 5): * p < 0.05, ** p < 0.01, *** p < 0.001.