Figure 4
Expression of Ym1 mRNA is significantly increased in the spinal cord of mSOD1/RAG2-/- mice. Quantitative RT-PCR analyses of spinal cord of mSOD1/RAG2-/- mice and mSOD1/RAG2+/+ mice at early (70-90 days of age) and end (130-150 days of age) stages were performed (n = 3-4 in each group). (A) Relative mRNA expression of M-CSF, IL-4, IL-4R, IL-13, IL-13R, Retnla, and Ym1, which are related to M2 macrophages, is shown. Ym1 expression levels are significantly increased in mSOD1-RAG2-/- mice at the early stage of disease (p = 0.039). (B) Relative mRNA expression of GM-CSF, IFN-γ, IFN-γ R, and NOS2, which are related to M1 macrophages, is shown. M1 macrophage-related molecules were comparable between mSOD1/RAG2-/- mice and control mice. (C) Relative mRNA expression of GDNF and SLC1A3 is shown. Expression levels of GDNF and SLC1A3 did not differ between mSOD1/RAG2-/- mice and control mice at early and end stages. Significance was evaluated using the student's t test. *: p < 0.05.