Leukocyte-endothelium interactions visualized using intravital microscopy. Rolling (A) and adhesion (B) of leukocytes in brain microvasculature were assessed in control mice and in mice infected with 4 × 103 PFU of DENV-3 at 3 and 6 days p.i. by the intracranial route. A higher number of rolling and adhering leukocytes was observed in the microvasculature in brain of infected mice. Data expressed as mean ± SEM of cells per minute (A) and per 100 μm (B). ***p < 0.001, **p < 0.01, *p < 0.05.