Figure 7

hSOD1 ^G93A rats show increased gene expression of Nox2, TLR4 and IL-1β at 21 days after PSNL. (A) mRNA levels of Nox2 (normalized for GAPDH mRNA expression) are higher in the ipsilateral dorsal quadrant of the lumbar spinal cord of transgenic rats at 21 days after PSNL, when compared to the other groups ($$$ p< 0.001; ## p< 0.01). (B) TLR4 gene expression was also increased in transgenic rats at 21 days after lesion when compared to their sham group ($$ p< 0.01) or the WT ligated group (## p< 0.01). (C) IL-1β transcript level was significantly higher in transgenic ligated rats at 21 days after lesion when compared to sham littermates ($$$ p< 0.001) or WT ligated rats (## p< 0.01). Each group included 5 rats, ANOVA and Tukey post-hoc test as statistical analyses.