Figure 8
IL-1β-induced C/EBPδ binding to a κB site is inhibited by Aβ. A) Representative western blot of C/EBPδ after streptavidin-agarose pull-down using biotinylated κB oligonucleotides. Primary astro-microglial cells were exposed to oligomer- or fibril-enriched preparations of Aβ (10 μM, oAβ or fAβ) in combination with IL-1β (10 ng/ml) for 3 h. κB oligonucleotides without biotin were used as a control for unspecific binding. B) Relative levels of C/EBPδ binding to the κB site showing that IL-1β induces a significant increase that is inhibited after Aβ exposure. The data represent mean ± SEM for three independent experiments. *p < 0.05, **p < 0.01, significantly different from control or otherwise indicated.