Figure 5

MyD88 signaling regulates inflammatory mediator production by microglia and infiltrating immune cells associated with CNS C. koseri infection. Viable microglia (A), macrophages (B), and neutrophils (C) were recovered from lesions of MyD88 KO and WT mice at 24 h following C. koseri infection by FACS based on exclusion of the vital dye 7-AAD. Cells were cultured overnight without additional stimulation and conditioned supernatants were analyzed for mediator expression by multi-analyte microbead arrays. The amount of each cytokine/chemokine was normalized based on total cell numbers and represents the mean ± SEM from three independent experiments.