Figure 8
MyD88-dependent signals play a pivotal role in mediating astrocytic inflammatory mediator release in response to C. koseri. Primary astrocytes recovered from MyD88 WT or KO mice were plated at 1 × 105 cells/well in 96-well plates and exposed to heat-inactivated C. koseri (107 cfu/well) for 24 h. The levels of NO (A) and CXCL2 (B) produced by astrocytes were quantitated by nitrite assay and ELISA, respectively. Astrocyte viability was assessed by a standard MTT assay and the raw OD570 absorbance values are reported (C). Significant differences in inflammatory mediator expression between C. koseri stimulated MyD88 KO and WT astrocytes are denoted by asterisks (* p < 0.05; ** p < 0.001, t-test). Results are reported as the mean ± SD of three independent wells for each experimental treatment and were identical across three separate experiments.