Figure 1

Expression and activation of various PKC isoforms in BV-2 cells. A, RNA samples isolated from the BV-2 cells were analyzed by quantitative real-time PCR, using primer pairs (see table 1) specific for mouse PKC α, β, γ, δ, ε, θ, η, ζ and λ. GAPDH was used as an internal control. A representative experiment of three that were performed is shown. B, Twenty micrograms of whole cell lysate from BV-2 cells for the detection of each PKC isoform was subjected to western blots with antibodies against PKC α, β, γ, δ, ε, θ, η, ζ and λ. A representative experiment of three that were performed is shown. C, BV-2 cells were treated with LPS (1 μg/ml) in the absence or presence of PKC inhibitors for 30 min and then lysed for assaying the PKC activity. CON, RO, GO and BIS stand for control, rottlerin, GO6976 and bisindolylmaleimide-1, respectively. All the PKC inhibitors completely blocked LPS-induced increases in PKC activity in BV-2 cells. **, p < 0.01 were obtained when the LPS alone-treated group was compared to the control and the drug-treated groups. A representative experiment of three that were performed is shown.