p38 phosphorylation acts as the break for CoMTb suppression of MMP-2 secretion. Microglial cells were incubated with SB203580 or PD98059 for 2 h, and then stimulated with CoMTb. MMP-2 secretion was analyzed at 72 h by Luminex and gelatin zymography (representative gel shown). p38 inhibition reverses CoMTb suppression and leads to supra-maximal MMP-2 secretion but ERK inhibition further suppresses secretion. Bars represent mean values ± SD of three samples, representative of at least duplicate experiments performed in triplicate. Data were analyzed by one-way analysis of variance, followed by Tukey's multiple comparison. **p < 0.01.