Level of VEGF-A in serum (A) and CSF (B) of definite ALS, probable ALS, possible ALS patients and normal subjects. Boxes include values from first quartile (25th percentile) to third quartile (75th percentile). Lower and upper error bar refers to 10th and 90th percentile respectively. The thick horizontal line in the box represents median for each dataset. Levels of VEGF-A were normalized to total protein in the serum and CSF samples. Outliers and extreme values are shown in circles and asterisk respectively. # indicates significant difference (p < 0.05) between the given conditions. Data was analyzed by Mann Whitney U Test. ALS, Amyotrophic Lateral Sclerosis; VEGF-A, vascular endothelial growth factor-A; pg, picogram; μg, microgram.