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Figure 2 | Journal of Neuroinflammation

Figure 2

From: Lentiviral vector-mediated stable expression of sTNFR-Fc in human macrophage and neuronal cells as a potential therapy for neuroAIDS

Figure 2

Photomicrographs of lentiviral vector transudced CHME and HTB cells showing GFP expression. CHME and HTB cells at exponential growth phase were transduced by the lentiviral vector at an MOI of 10 and transduction efficiency was evaluated by counting the percentage of GFP-positive cells within a transduced cell population using a fluorescence microscope (Nikon Eclipse TE2000-U). Photomicrographs showing transduced cells under phase contrast (PC) bright light or fluorescent light (FL) at 488 nm were taken at magnifications of 100× originally and scale bars in the pictures denote 100 μm. CHME-NT = non-transduced normal CHME cells; CHME-T1 = one transduction and CHME-T2 = two transductions; HTB-NT = non-transduced HTB cells and HTB-T = transduced HTB cells.

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