Figure 5

Transduction with lentivirus vectors does not result in inflammatory activation of CHME-5 or HTB-11 cells. Endogenous TNF-α secretion into the conditioned media of cells was measured by ELISA. Cells tested were as follows: CHME-NT, HTB-NT: non-transduced CHME-5 or HTB-11 cells; CHME-Fc, HTB-Fc: CHME-5 or HTB-11 cells transduced with the empty vector expressing Fc fragment. Results shown represent mean levels of TNF-α expression from three independent experiments and error bars denote the standard deviation.