Double-label immunofluorescence confirm Axl-/- (b,d) mice have fewer Iba1+ microglia/macrophages than WT (a,c) mice in ventral (a,b), lateral and dorsal funiculi (c,d). Ten micron frozen spinal cord sections were stained with an Iba1 pAb (red), a CD3 mAb (green), counterstained with Hoechst stain (blue), and visualized by immunofluorescence. a,b) Left panel shows merged triple fluorescence, middle panel shows CD3/Hoechst, and right panel shows Iba1/Hoechst for the ventral funiculus of WT (a) and Axl-/- (b) spinal cords. c,d) Merged images of lateral and dorsal funiculi for WT and Axl-/- spinal cords. All images are 40×. e) CD3+/Iba1+ cells/lesion for WT and Axl-/- mice.