Table 1 Sequences for primers and probes employed in this study
GENES | PRIMER/PROBE | SEQUENCES FOR PRIMERS AND PROBES |
|---|---|---|
18S | sense | AAATCAGTTATGGTTCCTTTGGTC |
antisense | GCTCTAGAATTACCACAGTTATCCAA | |
Probe #55 | Use universal ProbeLibrary, Roche Applied Science | |
iNOS | sense | GCTCCTCCCAGGACCACA |
antisense | GCTGGAAGCCACTGACACTT | |
Probe TaqMan | 6FAM-CACCTACCGCACCCGAGATGG--BBQ | |
COX-2 | sense | TGACCCACTTCAAGGGAGTCT |
antisense | CTGTCAATCAAATATGATCTGGATGTC | |
Probe TaqMan | 6FAM-AACAACATCCCCTTCCTGCGAAGTT--BBQ | |
TNF-α | sense | GCCTATGTCTCAGCCTCTTCTCATT |
antisense | CCACTTGGTGGTTTGCTACGA | |
Probe TaqMan | 6FAM-CCATAGAACTGATGAGAGGGAGGCCATTT-BBQ |