Cytokines and transcription factors in mouse cells after 1,25(OH)2D3 treatment. A) ELISA results for T cells activated with increasing concentrations of mouse anti-CD3 demonstrate increasing IFNγ and IL-5 concentrations, but 1,25(OH)2D3 suppresses the IFNγ concentration while increasing the IL-5 concentration. This results in an increase in the IL-5 to IFNγ ratio across all concentrations of anti-CD3. Each bar is the mean ± SD of quadruplicate cultures and data displayed is from a single mouse, repeated 3 times. B) qPCR results for 100ng/mL anti-CD3 demonstrate decreased IFNγ and IL-17 mRNA and increased IL-5 mRNA for increasing concentrations of 1,25(OH)2D3. C) The ratio of IL-5/IFNγ and IL-5/IL-17 mRNA rises with increasing concentrations of 1,25(OH)2D3. Similarly, the ratio of mRNA for GATA-3/T-bet and GATA-3/ROR-γT as determined by qPCR elevates with increasing concentrations of 1,25(OH)2D3.