Figure 6
Determination of cytokine expression after stroke in time and the effect of hypothermia. Effect of hypothermic treatment on the protein levels of cytokines as a function of time after Et-1 administration for sham, normothermic and hypothermic rats. Levels are expressed as pg/mg protein and are shown as mean Ā± SEM. Graphs (A) and (B) show the levels of IL-1Ī² at several time points after the administration of Et-1 for striatum and cortex respectively, (C) and (D) for TNF-Ī± and (E) represent the levels of TGF-Ī² in the cortex. The number of rats used to determine the levels for IL-1Ī² and TGF-Ī² were in the sham group: 6, 4 and 4 at 8, 24 and 72 hours; in the normothermic group: 6 at 8, 24 and 72 hours; and in the hypothermic group: 6, 6 and 5 at 8, 24 and 72 hours. The number of rats used to determine the levels for TNF-Ī± was in the sham group: 6 at 8 and 24 hours; in the normothermic group: 5 and 6 at 8 and 24 hours; and in the hypothermic group: 5 and 6 at 8 and 24 hours. Basal levels were measured in rats that underwent no treatment (n = 10) *Significantly different (p < 0.05) from basal levels determined by one-way ANOVA with Dunnett post-hoc test. Ā§Significantly different (p < 0.05) from normothermic rats as determined by one-way ANOVA with Dunnett post-hoc test.